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Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody, DyLight 649 (Pre-adsorbed), Novus Biologicals™
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Catalog No. NBP151902 Shop All R&D Systems Products
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Description

Goat Polyclonal Antibody

IgG F(ab')2 Polyclonal antibody specifically detects IgG F(ab')2 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

Antigen IgG F(ab')2
Applications Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration 0.5 mg/ml
Conjugate DyLight 649
Dilution Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Gene Symbols IGHG1
Host Species Goat
Immunogen Human IgG-F(ab')2 Fragment
Purification Method Affinity Purified
Quantity 1 mL
Regulatory Status RUO
Research Discipline Epitope Tags
Primary or Secondary Secondary
Gene ID (Entrez) 3500
Test Specificity Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.
Target Species Human
Content And Storage Store at 4C. Do not freeze.
Isotype IgG
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For Research Use Only

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