Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody (Pre-adsorbed), Novus Biologicals™

Catalog No.	Quantity
NBP261929	1 mL

Catalog No. NBP261929 Supplier Novus Biologicals Supplier No. NBP261929

Description

Goat Polyclonal Antibody

IgG F(ab')2 Polyclonal antibody specifically detects IgG F(ab')2 in Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry.

Specifications

• Antigen: IgG F(ab')2
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1:1000 - 1:20000, ELISA 1:1000 - 1:20000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry 1:200 - 1:2000
• Formulation: Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide
• Host Species: Goat
• Immunogen: Mouse IgG-F(ab')2 Fragment
• Purification Method: Affinity Purified
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
• Target Species: Mouse
• Content And Storage: Store at 4C. Do not freeze.
• Isotype: IgG