Invitrogen™ GPNMB Recombinant Mouse Monoclonal Antibody (HOST5DS), Alexa Fluor™ Plus 647

Catalog No.	Quantity
74-004-1MP647	50 μL
50-299-2670	20 μL

Catalog No. 74-004-1MP647 Supplier Invitrogen™ Supplier No. 740041MP647

Description

Mouse Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This HOST5DS antibody has been pre-titrated and tested by flow cytometric analysis of human blood monocyte-derived macrophages. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Tissue dissociation protocols involving enzymatic digestion should be optimized to limit proteolytic degradation of GPNMB on the cell surface. Excitation:...

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: GPNMB
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: HOST5DS
• Concentration: 1.0 mg/mL
• Conjugate: Alexa Fluor Plus 647
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene: GPNMB
• Gene Accession No.: Q14956
• Gene Alias: Dchil; DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; Gpnmb; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; Hgfin; ipd; iris pigment dispersion; NMB; Osteoactivin; si:ch211-155k24.6; transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB; Unknown (protein for MGC:133733); UNQ1725/PRO9925
• Gene Symbols: GPNMB
• Host Species: Mouse
• Immunogen: Fc-tagged full protein expressed in insect cells.
• Purification Method: Protein A/G
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10457
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ