Invitrogen™ Mouse GM-CSF, Recombinant Protein

Description

Recombinant Protein

Granulocyte Macrophage-CSF (mouse) Recombinant Protein, RP-8620, from Invitrogen.

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Recombinant mouse GM-CSF is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 14.3 kDa. Purity is typically greater than 95% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse FDCP-1 cell line and is typically less than 10 pg/mL. The specific activity of this protein is 1x10^8 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK.

Specifications

• Accession Number: P01587
• Concentration: 0.1mg/mL
• For Use With (Application): Control
• Formulation: 0.01M acetic acid with no preservative
• Gene ID (Entrez): 12981
• Name: Mouse GM-CSF
• Quantity: 20 μg
• Source: E. Coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: M-GM-CSF
• Common Name: Mouse GM-CSF
• Gene Symbol: Csf2
• Specific Reactivity: Mouse
• Conjugate: Unconjugated
• Cross Reactivity: Ms
• Species: E. coli
• Recombinant: Recombinant
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified