Invitrogen™ Granulysin Monoclonal Antibody (eBioDH2 (DH2)), PE, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 12882842

Catalog No. 50-112-18

Description

Description: The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells. Applications Reported: This eBioDH2 (DH2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry. Applications Tested: This eBioDH2 (DH2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

GNLY is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. GNLY is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms.

Specifications

• Antigen: Granulysin
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P22749
• Gene Symbols: GNLY
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10578
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioDH2 (DH2)
• Conjugate: PE
• Gene: GNLY
• Gene Alias: D2S69E; GNLY; Granulysin; LAG2; LAG-2; lymphocyte-activation gene 2; Lymphokine; lymphokine LAG-2; Lymphokine NKG5519; NKG5; Protein NKG5; T-cell activation protein 519; TLA519; T-lymphocyte activation gene 519
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ