Invitrogen™ GSDMD Recombinant Rabbit Monoclonal Antibody (PD00-18)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA550686

Catalog No. FEMA550686

Description

Positive control: PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours and then treated with LPS for 6 hours, PC-12 cell lysate, PMVEC cell lysate, RH-35 cell lysate, L6 cell lysate. Predicted band size: 53/30 kDa Subcellular Location: Cell membrane, Secreted.

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

Specifications

• Antigen: GSDMD
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P57764, Q9D8T2
• Gene Symbols: GSDMD
• Immunogen: Recombinant protein within Gasdermin D full length protein aa 1-484/484.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: PD00-18
• Conjugate: Unconjugated
• Gene: GSDMD
• Gene Alias: 1810036L03Rik; AW558049; DF5L; Dfna5l; FKSG10; gasdermin D; gasdermin domain containing 1; gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; GSDMD; GSDMDC1; GSDMD-CT; GSDMD-NT; M2-4
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 315084, 69146, 79792
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid