Cytiva GST GraviTrap™ Column

Description

For purification of fusion proteins

• Contains ten disposable columns prepacked with 2mL of Glutathione Sepharose 4B
• Convenient, prepacked gravity-flow columns
• Allows direct purification of both clarified and unclarified cell lysates
• Yields up to 20mg of pure proteins per column
• GST-tagged proteins are recovered under mild elution conditions (10mM glutathione), which preserves functionality of the proteins
• Binding capacity of prepacked GST GraviTrap columns varies with the nature of the tagged protein and the exact binding conditions used

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Specifications

• Bed Volume: 2 mL
• Binding Capacity: Up to 20 mg recombinant glutathione S-transferase (rGST)
• Diameter (English) Inner: 0.59 in.
• Diameter (Metric) Inner: 15 mm
• Description: GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein
• Material: Polypropylene
• pH Range: 4 to 13
• Support Type: 4% Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 45 to 165 μm
• Quantity: 10 x 2 mL
• For Use With (Application): For fast and simple manual purification of GST-tagged proteins by gravity flow
• Type: Gravity Flow Column