Cytiva GSTPrep™ FF 16/10 Column

Description

Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins

Glutathione Sepharose™ 4 Fast Flow is prepacked in 20mL GSTPrep columns for purification of GST-tagged proteins (as well as glutathione S-transferases and glutathione-dependent proteins). Mild elution conditions preserve protein antigenicity and function.

Features:

• Binds approx. 200mg recombinant GST/column
• Simple operation with pump or chromatography system (e.g., AKTA™)

GSTPrep FF 16/10 Prepacked Columns

• Volume: 20mL
• Dimensions: 1.6 x 10cm
• Recommended flowrate (H₂O at room temp): 1 to 10mL/min. (30 to 300cm/hr.)
• Max. flowrate (H₂O at room temp): 10mL/min. (300cm/hr.)
• Max. pressure during operation (H₂O at room temp): 1.5bar (0.15MPa, 22psi)
• Hardware pressure limit: 5bar (0.5MPa, 73psi)
• Storage/temperature: 20% ethanol/4° to 30°C

Specifications

• Buffer: 1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride
• Product Type: GSTPrep Glutathione Sepharose 4 FF column
• Bed Volume: 20mL
• Diameter (English) Inner: 0.62 in.
• Diameter (Metric) Inner: 16mm
• Quantity: 1 x 20 mL
• Height (English): 3.9 in.
• Height (Metric): 100mm
• Flow Rate: <10mL/min.
• Ligand Type: Glutathione and 10 Carbon linker arm
• Max. Pressure: 1.5 bar
• pH Range: 3 to 12
• Particle Size: 90μm
• Support Type: 4% Highly Cross-linked Agarose
• Content And Storage: 4° to 30°C
• Column Type: Ion Exchange Column
• For Use With (Application): Purification of Glutathione S-transferases and Glutathione-dependent Proteins