Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Description

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

• Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
• May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
• Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
• Average particle size: 90μm
• 1 and 5mL columns

Media

• Matrix: Highly cross-linked 4% agarose
• Binding capacity: >25mg horse liver GST/mL
• pH stability working range: 4 to 13

1mL columns

• Bed dimensions: 7 × 25mm
• Flow velocity: <4mL/min.

Specifications

• Bed Volume: 1 mL
• Diameter (English) Inner: 0.27 in.
• Diameter (Metric) Inner: 7 mm
• Description: GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
• Flow Rate: <4 mL/min.
• Material: Polypropylene
• pH Range: 4 to 13
• Support Type: 4% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Particle Size: 90 μm
• Quantity: 5 x 1 mL
• For Use With (Application): GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
• Type: Pre-packed Column
• Ligand Type: Glutathione