Cytiva GSTrap™ FF Columns: 16 x 25mm

Description

Purifies GST-tagged proteins in one step

• Mild elution conditions preserve antigenicity and function of proteins extracted
• Prepacked with Glutathione Sepharose™ media
• Average particle size: 90μm
• Bed dimensions: 16 × 25mm
• Flow velocity: <20mL/min.

Media

• Matrix: Highly cross-linked 4% agarose
• Ligand: glutathione and 10-carbon linker arm
• Binding capacity: >10mg/mL
• pH stability working range: 3 to 12

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Specifications

• Bed Volume: 5 mL
• Diameter (English) Inner: 0.62 in.
• Diameter (Metric) Inner: 16 mm
• Description: GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
• Flow Rate: <20 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 4% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Particle Size: 90 μm
• Quantity: 5 x 5 mL
• For Use With (Application): For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
• Type: Pre-packed Column
• Ligand Type: Glutathione and 10 carbon linker arm