Cytiva GSTrap™ HP Columns

Catalog No.	Quantity
45-000-334	5 x 5 mL
45-000-332	5 x 1 mL
45-000-333	1 x 5 mL

Catalog No. 45-000-334

Supplier: Cytiva 17528202

Description

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

• One-step purification of GST-tagged proteins and other glutathione S-transferases or glutathione-dependent proteins
• Glutathione coupled to Sepharose™ High Performance media
• Column material: polypropylene
• May be used with syringe, peristaltic pump or chromatography system
• Purification process preserves antigenicity and function
• Available as 1mL and 5mL columns

Media

• Average particle size: 34μm
• Ligand: Glutathione
• Matrix: Highly cross-linked 6% agarose
• Binding capacity: >7mg/mL
• pH stability working range: 3 to 12

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Bed Volume: 5 mL
• Diameter (English) Inner: 0.62 in.
• Diameter (Metric) Inner: 16 mm
• Description: GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
• Flow Rate: <20 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 4% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Particle Size: 34 μm
• Quantity: 5 x 5 mL
• For Use With (Application): Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
• Type: Pre-packed Column
• Ligand Type: Glutathione