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  6. Invitrogen™ H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified

Invitrogen™ H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified
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Catalog No. PIMA518067
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100 μL
20 μg
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PIMA518067 100 μL
PIA51806720 20 μg
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Catalog No. PIMA518067 Supplier Invitrogen™ Supplier No. MA518067
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Description

Mouse Monoclonal Antibody

This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone 3 or tri-, di-, or unmethylated histone H4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogast), and most mammals based on sequence homology. This target has a predicted molecular weight of 11 kDa. Actual Western blot results show a band at 13 kDa. This can be blocked using mono-methyl K20 Histone H4 peptide. Click here for Master Lot linking information.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
TRUSTED_SUSTAINABILITY

Specifications

Antigen H4K20me1
Applications ChIP Assay, Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 5E10-D8
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% sodium azide
Gene H4C1
Gene Accession No. P62805
Gene Alias BcDNA:RH52884; CELE_T10C6.11; CG31611; CG3379; CG3379-PA; CG3379-PB; CG3379-PC; CG3379-PD; CG33869; CG33871; CG33873; CG33875; CG33877; CG33879; CG33881; CG33883; CG33885; CG33887; CG33889; CG33891; CG33893; CG33895; CG33897; CG33899; CG33901; CG33903; CG33905; CG33907; CG33909; Dmel\CG3379; Dmel_CG3379; F07B7.11; F07B7.4; F45F2.12; FBtr0082962; FO108; H4; H4 clustered histone 11; H4 clustered histone 2; H4 histone family, member A; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20; H4.1; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4-12; H4-16; H4-53; H4C1; H4C11; H4C12; H4C13; H4c14; H4C15; H4C2; H4c3; H4c4; H4C5; H4c6; H4c8; H4C9; H4f16; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4K20; H4K20me; H4Lys20me; H4Lys20me1; H4M; H4r; his-20; his-22; His4; his-4; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; His4-88CD; His4r; His4r-PA; His4r-PB; His4r-PC; His4r-PD; his-52; his-54; his-8; HIST1H4; hist1h4a; hist1h4a.L; Hist1h4b; Hist1h4c; Hist1h4d; HIST1H4E; Hist1h4f; HIST1H4H; HIST1H4I; HIST1H4J; Hist1h4k; HIST1H4L; Hist1h4m; Hist2h4; HIST2H4A; HIST2H4B; HIST4H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4l; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1, H4a; histone cluster 1, H4a L homeolog; histone cluster 1, H4b; histone cluster 1, H4e; histone cluster 1, H4i; histone cluster 2, H4; histone cluster 2, H4a; Histone Cluster 4; histone cluster 4, H4; histone family member; Histone H2B 2; histone H3; histone H4; Histone H4 replacement; histone H4.1; Histone H4A; Histone H4K20me1; histone IV, family 2; K06C4.12; K06C4.4; LOC102161782; methyl Histone 3; methyl Histone H3; Methyl-H4 K20; Methyl-H4 Lys20; Methyl-Histone H4 K20; Methyl-Histone H4 Lys20; Mono-methyl H4; Mono-methyl Histone H4; RH52884p; T10C6.11; X04652; XELAEV_18001418mg
Gene Symbols H4-16, H4C1, H4C11, H4C14, H4C2, H4C5, H4C9
Host Species Mouse
Immunogen Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched)).
Purification Method Protein G
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 121504, 8294, 8359, 8363, 8366, 8367, 8370
Target Species Human
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG1
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