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CD279 Hamster anti-Mouse, APC-R700, Clone: J43, BD

Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 565815

Catalog No. BDB565815


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Description

Description

The J43 monoclonal antibody specifically binds to CD279 (PD-1), a 50-55-kDa glycoprotein encoded by the Pdcd1 gene of the CD28 family of the Ig superfamily. The expression of Pdcd1 mRNA and PD-1 protein is tightly regulated. PD-1 is transiently expressed on CD4-CD8thymocytes, it is upregulated on some cell lines upon induction of apoptosis, it is induced on thymocytes and splenic T and B lymphocytes after stimulation through their antigen receptors, and it is induced on activated myeloid cells. In addition, Pdcd1 mRNA is transiently expressed in developing B lymphocytes at the pro-B-cell stage. The presence of an ITIM ( yrosine-based I nhibitory M otif) on PD-1's intracytoplasmic region and the development of splenomegaly and breakdown of peripheral tolerance in PD-1[-/-] mice suggest that PD-1 may be involved in the negative regulation of immune responses. The PD-1 ligands, B7-H1 (also known as PD-L1) and B7-DC (PD-L2), are members of the B7 family of the Ig superfamily. The J43 antibody blocks the binding of PD-1 to its two ligands.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

Specifications

CD279
J43
APC-R700
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Hamster
IgG2, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
PD-1; Pdcd1; Pd1; Pdc1; Ly101; programmed cell death 1
Affinity Purified
Syrian Hamster kidney cell line BKH transfected with Pdcd1 cDNA
Affinity Chromatography
RUO
Primary
Murine
Documents
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