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CD279 Hamster anti-Mouse, APC-R700, Clone: J43, BD

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Description

Hamster Monoclonal Antibody

The J43 monoclonal antibody specifically binds to CD279 (PD-1), a 50-55-kDa glycoprotein encoded by the Pdcd1 gene of the CD28 family of the Ig superfamily. The expression of Pdcd1 mRNA and PD-1 protein is tightly regulated. PD-1 is transiently expressed on CD4-CD8thymocytes, it is upregulated on some cell lines upon induction of apoptosis, it is induced on thymocytes and splenic T and B lymphocytes after stimulation through their antigen receptors, and it is induced on activated myeloid cells. In addition, Pdcd1 mRNA is transiently expressed in developing B lymphocytes at the pro-B-cell stage. The presence of an ITIM ( yrosine-based I nhibitory M otif) on PD-1's intracytoplasmic region and the development of splenomegaly and breakdown of peripheral tolerance in PD-1[-/-] mice suggest that PD-1 may be involved in the negative regulation of immune responses. The PD-1 ligands, B7-H1 (also known as PD-L1) and B7-DC (PD-L2), are members of the B7 family of the Ig superfamily. The J43 antibody blocks the binding of PD-1 to its two ligands.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD279
Applications Flow Cytometry
Classification Monoclonal
Clone J43
Concentration 0.2mg/mL
Conjugate APC-R700
Description PD-1; Pdcd1; Pd1; Pdc1; Ly101; programmed cell death 1
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Host Species Hamster
Immunogen Syrian Hamster kidney cell line BKH transfected with Pdcd1 cDNA
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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