Molecular Probes™ HCS NuclearMask™ Stains

Description

Easily identify cell nuclei and other features of interest during high-content screening (HCS) assays with the versatile HCS NuclearMask Blue, Red, and Deep Red stains, which can be used with live and fixed cells and in multiparametric analyses.

Easily identify cell nuclei in high-content screening (HCS) assays with the versatile HCS NuclearMask Blue, Red, and Deep Red stains, which survive standard formaldehyde-based fixation and detergent-based permeabilization methods and can be applied to live and fixed cells. Because their dyes are easily discriminated from other fluorophores, the HCS NuclearMask stains can be used in multiparametric analyses. They can also label the entire cell (i.e., cytoplasm and nucleus) and provide an accurate backdrop against which to assess the features of interest.

The versatile HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclearMask Deep Red stains can be used to measure DNA content and perform cell demarcation with formaldehyde-fixed cells on high content imaging and analysis (HCS) platforms. HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclarMask Deep Red stains have excitation/emission maxima of 355/460 nm, 622/645 nm, and 638/686 nm, respectively, when bound to DNA. HCS NuclearMask Blue stain is easily discriminated from visible- and near-IR wavelength fluorophores, HCS NuclearMask Red stain is easily discriminated from green- and even orange-fluorescent fluorophores, and HCS NuclearMask Deep Red is easily discriminated from visible wavelength fluorophores when used in multiparametric analyses.

If total cell staining is desired, HCS CellMask stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask stains are available in a choice of colors for multiplexing flexibility. Sufficient volume quantities are provided to stain ten 96-well plates using an assay volume of 100 μL per well.

Specifications

• Color: Blue
• Content And Storage: Includes 65 μL of 2000X concentrate, providing sufficient material to stain a total of ten 96-well microplates.
  
  Store at 2–6°C and protect from light.
  
  
• Excitation Wavelength Range: 350/451
• For Use With (Equipment): High Content Instrument
• Quantity: 65 μL
• Detection Method: Fluorescence
• Description: HCS NuclearMask™ Blue Stain
• Emission: Visible
• Product Line: NuclearMask™
• Shipping Condition: Room Temperature
• Label Type: Fluorescent Dye
• Product Type: Stain
• SubCellular Localization: Nucleic Acids, Nucleus