Invitrogen™ HDAC2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1861

Catalog No. PA1861

Description

PA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells. PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining. The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: HDAC2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HDAC2
• Gene Alias: D10Wsu179e; HD2; hdac 2; Hdac2; hdac-2; Histone deacetylase 2; histone deacetylase 2-like protein; histone deacetylase-2; I79_010219; mRPD3; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1 transcription factor-binding protein; YY1-associated factor 1; Yy1bp
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100764557, 15182, 3066, 475035, 84577
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P70288, Q92769
• Gene Symbols: HDAC2
• Immunogen: Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Monkey, Hamster
• Product Type: Antibody
• Isotype: IgG