Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. PIMA545242

Invitrogen™ HDEL Monoclonal Antibody (2E7), PerCP
GREENER_CHOICE

Catalog No. PIMA545242
Change view
Click to view available options
Quantity:
100 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PIMA545242 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Catalog No. PIMA545242 Supplier Invitrogen™ Supplier No. MA545242
Only null left
Add to Cart
Add to Cart
This item is not returnable. View return policy

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-45242 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78kDa.

HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen HDEL
Applications Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 2E7
Concentration 1 mg/mL
Conjugate PerCP
Formulation 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
Gene KdelR
Gene Accession No. O76767
Gene Alias BEST:CK00230; CG5183; CG5183-PA; CG5183-PB; CG5183-PC; CK00230; Dmel\CG5183; Dmel_CG5183; dmErd2; ER lumem protein retaining receptor 2; ER lumen protein retaining receptor; ER lumen protein retaining receptor 2; ER lumen protein-retaining receptor; Erd2; KDEL receptor; KdelR; KDELR1; KDEL-receptor; KdelR-PA; KdelR-PB; KdelR-PC; l(2)31Em; l(2)k00311; lethal (2) 31Em
Gene Symbols KdelR
Host Species Mouse
Immunogen Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 34427
Target Species Drosophila, Yeast, Plant
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2b
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.