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Invitrogen™ HDEL Monoclonal Antibody (2E7), PerCP
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Catalog No. PIMA545242
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Quantity:
100 μg
Catalog No. Quantity
PIMA545242 100 μg
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Catalog No. PIMA545242

Supplier: Invitrogen™ MA545242

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Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-45242 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78kDa.

HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen HDEL
Applications Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 2E7
Concentration 1 mg/mL
Conjugate PerCP
Formulation 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
Gene KdelR
Gene Accession No. O76767
Gene Alias BEST:CK00230; CG5183; CG5183-PA; CG5183-PB; CG5183-PC; CK00230; Dmel\CG5183; Dmel_CG5183; dmErd2; ER lumem protein retaining receptor 2; ER lumen protein retaining receptor; ER lumen protein retaining receptor 2; ER lumen protein-retaining receptor; Erd2; KDEL receptor; KdelR; KDELR1; KDEL-receptor; KdelR-PA; KdelR-PB; KdelR-PC; l(2)31Em; l(2)k00311; lethal (2) 31Em
Gene Symbols KdelR
Host Species Mouse
Immunogen Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 34427
Target Species Drosophila, Yeast, Plant
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2b
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