Novus Biologicals™ HE4/WFDC2 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HE4/WFDC2.

Specifications

• Assay Range: 15.625 pg/mL
• Assay Sensitivity: 15.625-1000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HE4/WFDC2.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: dJ461P17.6, EDDM4, Epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, HE4MGC57529, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WAP domain containing protein HE4-V4, WAP four-disulfide core domain 2, WAP four-disulfide core domain protein 2, WAP5epididymal protein 4
• Gene ID (Entrez): 10406
• Gene Symbol: WFDC2
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 50 ng of recombinant Human HE4/WFDC2.
  Capture Antibody: 1 mg/mL of mouse anti-Human HE4/WFDC2 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human HE4/WFDC2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer
• Storage Requirements: Storage is content dependent.
• Target: HE4/WFDC2
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.05 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.