Invitrogen™ HELIOS Monoclonal Antibody (22F6), PE, eBioscience™

Catalog No.	Quantity
50-112-2489	100 Tests

Catalog No. 50-112-2489 Supplier Invitrogen™ Supplier No. 12988342

Description

Armenian Hamster Monoclonal Antibody

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia. 22F6 has been reported to cross react with rat Helios. Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse lymph node cell using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number...

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Specifications

• Antigen: HELIOS
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 22F6
• Concentration: 5 μL/Test
• Conjugate: PE
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: IKZF2
• Gene Accession No.: P81183, Q9UKS7
• Gene Alias: A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2
• Gene Symbols: IKZF2
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 22779, 22807, 488510
• Target Species: Human, Mouse, Canine
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov