Novus Biologicals™ Hemopexin Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human Hemopexin.

Specifications

• Assay Range: 18.8 pg/mL
• Assay Sensitivity: 18.8-1200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human Hemopexin.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: beta-1B-glycoprotein, FLJ56652, hemopexin, HX
• Gene ID (Entrez): 3263
• Gene Symbol: HPX
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.4 mg/mL of rabbit anti-Human Hemopexin polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4).
  Capture Antibody: 1 mg/mL of mouse anti-Human Hemopexin monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 48 ng of recombinant Human Hemopexin.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Angiogenesis, Cancer, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, HIF Target Genes, Hypoxia, Stem Cell Markers, Tyrosine Kinases
• Storage Requirements: Storage is content dependent.
• Target: Hemopexin
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.