Invitrogen™ Mouse HGF-Cell Lysates ELISA Kit

Description

ELISA

The Mouse Hepatocyte Growth Factor(Ms HGF) Cell Lysates ELISA quantitates Ms HGF in mouse cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

Specifications

• Accession Number: Q08048
• Assay Range: 0.614 to 60 ng/mL
• Assay Sensitivity: 400 pg/mL
• Calibration Range: 400 pg/mL to 60 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Chemokine & Receptors
• Sample Type: Cell Lysates
• Specificity: The antibody pair provided in this kit recognizes mouse HGF.
• Sufficient For: 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 15234
• Gene Symbol: C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Apoptosis
• Sample Volume: Cell Lysate 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF
• Target: HGF
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins