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Cytiva HiPrep™ IEX FF 16/10 Columns

Catalog No. 45002070 Shop All Cytiva Products
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Ligand Type:
Carboxymethyl
Diethylaminoethyl
Max. Pressure:
1.5 bar
5 bar
pH Range:
2 to 12
6 to 10
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Catalog No. Ligand Type Max. Pressure pH Range
45-002-070 Carboxymethyl 1.5 bar 6 to 10
45-002-066 Diethylaminoethyl 5 bar 2 to 12
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Catalog No. 45-002-070 Supplier Cytiva Supplier No. 28936542
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Description

Provide fast, reliable and reproducible preparative ion exchange separations

DEAE and CM Sepharose™ 6 Fast Flow are prepacked in 20mL HiPrep columns. Ideal for rapid enrichments during initial capture of proteins from starting material.

Features:

  • Combine good flowrates and high binding capacity
  • Simple-to-operate
  • Compatible with single-pump configurations and other chromatography systems (e.g., AKTA™)

Specifications (H2O at room temp.)

  • Dimensions: 1.6 x 10cm
  • Recommended flowrate: 2-10mL/min. (60-300cm/h)
  • Maximum flowrate: 10mL/min. (300cm/hr.)
  • Max. pressure over packed bed: 1.5bar (22psi, 0.15MPa)
  • Column hardware pressure limit: 5bar (73psi, 0.5MPa)
  • Storage: 20% ethanol (DEAE,CM), 0.2M sodium acetate in 20% ethanol (SP)
  • Storage temperature: 4° to 30°C

TRUSTED_SUSTAINABILITY

Specifications

Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Product Type HiPrep CM Sepharose FF column
Bed Volume 20mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16mm
Quantity 1 x 20mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate >300 cm/hr.
Material Polypropylene
Ligand Type Carboxymethyl
Max. Pressure 1.5 bar
pH Range 6 to 10
Particle Size 45 to 165μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Ion Exchange Column
For Use With (Application) Preparative separations of Proteins and other biomolecules using ion exchange (IEX) chromato graphy
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