Cytiva HiPrep™ IMAC FF 16/10 Column

Description

For optimizing purification of histidine-tagged proteins at laboratory scale

IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up. The medium consists of 90μm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled. This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein.

Specifications:
IMAC Sepharose 6 Fast Flow

• Matrix: highly crosslinked agarose, 6%
• Average particle size: 90μm
• Binding capacity (protein/ion dependent): approx. 40mg histidine-tagged protein/mL medium when charged with Ni²⁺
• Compatibility: stable in all commonly used buffers
• Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr. tested 2% SDS; 30 min. tested 30% 2-propanol
• Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term)
• Storage/Temperature: 20% ethanol; 4° to 30°C

HiPrep Prepacked Columns

• Volume: 20mL
• Dimensions: 1.6 x 10cm
• Max. pressure during operation: 1.5bar (0.15MPa, 22psi)
• Hardware pressure limit: 5bar (0.5MPa, 73psi)

Recommend flowrate (H₂O at room temp.): 2 to 10mL/min.

Specifications

• Buffer: 0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
• Product Type: HiTrap IMAC Sepharose 6 FF column
• Bed Volume: 20mL
• Diameter (English) Inner: 0.62 in.
• Diameter (Metric) Inner: 16mm
• Quantity: 1 x 20mL
• Height (English): 3.93 in.
• Height (Metric): 100mm
• Flow Rate: <10mL/min.
• Material: Polypropylene
• Max. Pressure: 1.5 bar
• pH Range: 3 to 12
• Particle Size: 45 to 165μm
• Support Type: 6% Cross-linked Agarose
• Content And Storage: 4° to 30°C
• Column Type: Ion Exchange Column
• For Use With (Application): IMAC and subsequent purification of Polyhistidine tagged Proteins