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  6. Cytiva His Mag Sepharose™ excel Chromatography Media

Cytiva His Mag Sepharose™ excel Chromatography Media

Catalog No. 45003016 Shop All Cytiva Products
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45-003-016 5 x 1 mL
45-002-369 10 x 1 mL
45-003-015 2 x 1 mL
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Catalog No. 45-003-016 Supplier Cytiva Supplier No. 17371221
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Description

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.

  • His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
  • The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
  • Simplify handling using visible and dense Sepharose-based magnetic beads.
  • Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
  • The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
  • The base matrix is based on Sepharose medium with magnetite incorporated.
  • His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.

TRUSTED_SUSTAINABILITY

Specifications

pH Range 2 to 12
Support Type Highly cross-linked spherical Agarose including Magnetite
Content And Storage 4°C to 30°C
Particle Size 63 μm
Quantity 5 x 1 mL
For Use With (Application) Designed to simplify small-scale purification and screening of His-tagged Proteins secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
Type Chromatography Media
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