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Cytiva His SpinTrap™ Spin Columns

Catalog No. 45002017 Shop All Cytiva Products
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Spin Column
Spin Column Kit
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Catalog No. Type
45-002-017 Spin Column Kit
45-001-427 Spin Column
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Catalog No. 45-002-017 Supplier Cytiva Supplier No. 28932171
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Description

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

 

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
  • Kit components (spin columns and buffer) can also be purchased separately

 

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

 

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

TRUSTED_SUSTAINABILITY

Specifications

Kit Contents 1 Pk. His SpinTrap (28-4013-53) and 1 Pk. His Buffer Kit (11-0034-00)
pH Range 2 to 14
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Quantity 1 Piece
Type Spin Column Kit
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