Cytiva His SpinTrap™ Spin Columns

Description

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

 

• Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
• One run in standard microcentrifuge takes approximatedly 10 min.
• Yields up to 750μg purified protein per column
• Allows direct purification of both clarified and unclarifed cell lysates
• Also useful for screening large numbers of small-scale bacterial lysates
• Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
• Kit components (spin columns and buffer) can also be purchased separately

 

Medium Characteristics:

• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• Stable (long-term) in the pH range 3-12
• Average particle size: 34μm

 

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

• Kit Contents: 1 Pk. His SpinTrap (28-4013-53) and 1 Pk. His Buffer Kit (11-0034-00)
• pH Range: 2 to 14
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Quantity: 1 Piece
• Type: Spin Column Kit