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His Tag Antibody, R&D Systems™
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Catalog No. MAB050100
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Catalog No. Quantity
MAB050100 100 μg
MAB050SP 25 μg
MAB050500 500 μg
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Mouse Monoclonal Antibody has been used in 44 publications

His Tag Monoclonal specifically detects His Tag in Multi-Species samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunoaffinity Purification, CyTOF-ready.

Specifications

Antigen His Tag
Applications Western Blot, Flow Cytometry, Affinity Purification, CyTOF
Classification Monoclonal
Clone AD1.1.10
Conjugate Unconjugated
Dilution Western Blot 0.2 ug/mL, Simple Western 10 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoaffinity Purification, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias 6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Host Species Mouse
Immunogen His-tagged peptide
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Multi-species
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Supernatant
Isotype IgG1
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