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Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
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Ligand Type:
Blue Sepharose
DEAE Sepharose
Ni Sepharose
Description
- Based on 6% highly cross-linked agarose with an average bead size of 90μm
- Processed flow properties and high loading capacities
- Bed height of 10cm is designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
- Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 90μm
- Flow velocity: <600cm/hr or <450cm/hr
- Material (Column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Blue Sepharose
- Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
- Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
- Prepacked with Nickel Sepharose 6 Fast Flow
- Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
- Prepacked with DEAE Sepharose Fast Flow
- Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

Specifications
Specifications
Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Product Type | HiScreen Ni Sepharose 6 FF column |
Bed Volume | 4.7 mL |
Diameter (English) Inner | 0.3 in. |
Diameter (Metric) Inner | 7.7 mm |
Quantity | 1 x 4.7mL |
Height (English) | 3.93 in. |
Height (Metric) | 100mm |
Flow Rate | <450 cm/hr. |
Material | Polypropylene |
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