Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Description

Used for method optimization and parameter screening

• Based on 6% highly cross-linked agarose with an average bead size of 90μm
• Processed flow properties and high loading capacities
• Bed height of 10cm is designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume gives fast results and minimal sample/buffer consumption
• Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
• Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

• Bed volume: 4.7mL
• Bed dimension: 7.7 × 100mm
• Matrix: 6% cross-linked agarose beads
• Average particle size: 90μm
• Flow velocity: <600cm/hr or <450cm/hr
• Material (Column hardware and column tube): polypropylene
• Column I.D.: 7.7mm

Blue Sepharose

• Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
• Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins

Ni Sepharose

• Prepacked with Nickel Sepharose 6 Fast Flow
• Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)

DEAE Sepharose

• Prepacked with DEAE Sepharose Fast Flow
• Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

Specifications

• Buffer: 0.1M HCl, 0.1M NaOH, 8M Urea
• Product Type: HiScreen Ni Sepharose 6 FF column
• Bed Volume: 4.7 mL
• Diameter (English) Inner: 0.3 in.
• Diameter (Metric) Inner: 7.7 mm
• Quantity: 1 x 4.7mL
• Height (English): 3.93 in.
• Height (Metric): 100mm
• Flow Rate: <450 cm/hr.
• Material: Polypropylene
• Ligand Type: Ni Sepharose
• Max. Pressure: 1.5 bar
• pH Range: 3 to 12
• Particle Size: 45 to 165μm
• Support Type: 6% Cross-linked Agarose
• Content And Storage: 4° to 30°C
• Column Type: Prepacked Fast Flow Column
• For Use With (Application): Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy