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Cytiva HiScreen™ Capto™ Phenyl (High Sub) Prepacked HIC Column

Catalog No. 45002549 Shop All Cytiva Products
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Catalog No. 45-002-549 Supplier Cytiva Supplier No. 28992472
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Description

Hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, used for method optimization and parameter screening

  • Highly cross-linked agarose matrix with average bead size of 75μm
  • Has good flow properties and high loading capacities
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Connect easily in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 × 100mm
  • Flow rate: 1.2 to 2.7mL/min.
  • Flow velocity: <600cm/hr.
Media
  • Average particle size: 75μm
  • Degree of substitution: 27μmol/mL gel
  • pH stability cleaning: 2 to 14
  • Pressure/Flow specification: 300kPa at 600cm/hr., 1m diameter column, 20cm bed height
  • Medium: BioProcess
  • pH stability working range: 3 to 13
  • Ligand: phenyl
  • Binding capacity/mL chromatography medium: 27mg BSA /mL (at QB10%)
Column
  • Bed support: filter
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

TRUSTED_SUSTAINABILITY

Specifications

Buffer Aqueous Buffer
Product Type HiScreen Capto Phenyl (high sub) column
Bed Volume 4.7mL
Diameter (English) Inner 0.3 in.
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate <600cm/hr.
Material Polypropylene
Ligand Type Phenyl (∼27μM/mL drained medium)
Max. Pressure 43.5psi
pH Range 3 to 13
Particle Size 75μm
Support Type Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type HIC Column
For Use With (Application) Optimization and parameter screening
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