Cytiva HiScreen™ Capto™ Phenyl (High Sub) Prepacked HIC Column

Description

Hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, used for method optimization and parameter screening

• Highly cross-linked agarose matrix with average bead size of 75μm
• Has good flow properties and high loading capacities
• Bed height of 10cm designed to allow method optimization and parameter screening
• Connect easily in series to achieve 20cm bed height
• Small bed volume gives fast results and minimal sample/buffer consumption
• Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity
• Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

• Bed volume: 4.7mL
• Bed dimension: 7.7 × 100mm
• Flow rate: 1.2 to 2.7mL/min.
• Flow velocity: <600cm/hr.

Media

• Average particle size: 75μm
• Degree of substitution: 27μmol/mL gel
• pH stability cleaning: 2 to 14
• Pressure/Flow specification: 300kPa at 600cm/hr., 1m diameter column, 20cm bed height
• Medium: BioProcess
• pH stability working range: 3 to 13
• Ligand: phenyl
• Binding capacity/mL chromatography medium: 27mg BSA /mL (at QB10%)

Column

• Bed support: filter
• Material (column hardware and column tube): polypropylene
• Column I.D.: 7.7mm

Specifications

• Buffer: Aqueous Buffer
• Product Type: HiScreen Capto Phenyl (high sub) column
• Bed Volume: 4.7mL
• Diameter (English) Inner: 0.3 in.
• Diameter (Metric) Inner: 7.7mm
• Quantity: 1 x 4.7mL
• Height (English): 3.93 in.
• Height (Metric): 100mm
• Flow Rate: <600cm/hr.
• Material: Polypropylene
• Ligand Type: Phenyl (∼27μM/mL drained medium)
• Max. Pressure: 43.5psi
• pH Range: 3 to 13
• Particle Size: 75μm
• Support Type: Highly Cross-linked Agarose
• Content And Storage: 4° to 30°C
• Column Type: HIC Column
• For Use With (Application): Optimization and parameter screening