Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Catalog Number	Mfr. No.	Product Type	Ligand Type	pH Range
45-002-529	Cytiva 28926978	HiScreen Capto Q IEX column	Quaternary Amine	2 to 12
45-002-530	Cytiva 28926979	HiScreen Capto S IEX column	Sulphonate group	4 to 12
45-002-533	Cytiva 28926982	HiScreen Capto DEAE IEX column	Diethylaminoethyl	2 to 9

Description

• Fast, efficient, and cost-effective capture and intermediate protein purification
• Ready-to-use columns for convenient process development optimization
• Highly cross-linked agarose with dextran surface extender matrix
• Bed height of 10cm is designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
• Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

Capto S: Strong cation (sulfonate group) exchange BioProcess™ medium
Capto Q: Strong anion (quartenary amine group) exchange BioProcess medium
Capto DEAE: Weak anion (diethylaminoethyl group) exchange BioProcess medium

Specifications

• Bed Volume: 4.7mL
• Diameter (English) Inner: 0.3 in.
• Quantity: 1 x 4.7mL
• For Use With (Application): Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
• Height (Metric): 100mm
• Max. Pressure: 3 bar
• Support Type: Highly cross-linked Agarose with Dextran surface extender

• Buffer: 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
• Diameter (Metric) Inner: 7.7mm
• Flow Rate: <700cm/hr.
• Height (English): 3.93 in.
• Material: Polypropylene
• Particle Size: 45 to 165μm