Cytiva™ HiScreen™ Column with MabSelect™ VL Protein L Resin

Description

These HiScreen™ columns contain Protein L resin for affinity capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.

As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.

Redesigned protein L ligand MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to Cytiva™ Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing.

• A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability
• High dynamic binding capacity for efficient capture of target molecules
• Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
• Provides good resolution for product-related impurities in the capture of bispecific antibodies
• Convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development

Specifications

• Binding Capacity: 70 mg Human IgG/mL Resin at 6 min. Residence Time, 60 mg Human IgG/mL Resin at 4 min. Residence Time
• Capacity (Metric): 4.7 mL
• Diameter (English) Inner: 0.3 in.
• Diameter (Metric) Inner: 7.7 mm
• Flow Rate: <300 cm/hr.
• Max. Operating Pressure: 3 bar
• Particle Size: 60 μm
• Product Line: HiScreen™ MabSelect™
• Quantity: 1 Column
• For Use With (Application): Antibody Affinity Chromatography
• Target: Antibody
• Type: Pre-packed Column
• Format: Chromatography Column
• Bed Height: 100 mm
• Column Type: Affinity