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Cytiva HiScreen Phenyl FF (High Sub)

HiScreen Phenyl FF (high sub) columns are prepacked with Phenyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.

Supplier:  Cytiva 28926988

Catalog No. 45-002-537

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  • Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.Two levels of substitution help find optimal selectivity and binding capacity for a given application.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.


HiScreen Phenyl FF (High Sub)
7.7 mm
1.5 bar [0.15 MPa] (22 psi)
HIC Column
Less than 450 cm/h
4° to 30°C, 20% Ethanol or 0.01M NaOH
Hydrophobic Interaction Chromatography




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