Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Catalog Number	Mfr. No.	Product Type	Ligand Type	pH Range
45-002-539	Cytiva 28950510	HiScreen Q Sepharose FF column	Quaternary Amine	2 to 12
45-002-541	Cytiva 28950513	HiScreen SP Sepharose FF column	Sulphopropyl	4 to 13

Description

• Ready-to-use column for convenient process development optimization and parameter screening
• Highly cross-linked agarose matrix with average bead size of 90μm
• Bed height of 10cm designed to allow method optimization and parameter screening
• 20cm bed height is acheived when it is connected in series
• Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

• Bed dimension: 7.7 × 100mm
• Matrix: 6% cross-linked agarose beads
• Average particle size: 90μm
• Max. flow velocity: 450cm/hr.
• Material (column hardware and column tube): polypropylene
• Column I.D.: 7.7mm

HiScreen Q FF

• Media:
• pH stability working range: 2 to 12
• pH stability cleaning: 2 to 14
• Ion exchanger type: strong anion exchanger

HiScreen SP FF

• Media:
• pH stability working range: 3 to 14
• pH stability cleaning: 4 to 13
• Ion exchanger type: strong cation exchanger

Specifications

• Buffer: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
• Diameter (English) Inner: 0.3 in.
• Quantity: 1 x 4.7mL
• Height (Metric): 100mm
• Material: Polypropylene
• Particle Size: 45 to 165μm
• Content And Storage: 4° to 30°C
• For Use With (Application): Optimization and parameter screening, ion exchange chromato graphy

• Bed Volume: 4.7mL
• Diameter (Metric) Inner: 7.7mm
• Height (English): 3.93 in.
• Flow Rate: <450cm/hr.
• Max. Pressure: 1.5 bar
• Support Type: 6% Cross-linked Agarose
• Column Type: Ion Exchange Column