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Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns

Catalog No. 45002540 Shop All Cytiva Products
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Product Type:
HiScreen Q Sepharose HP Column
HiScreen SP Sepharose HP Column
Ligand Type:
Quaternary Amine
Sulphopropyl
pH Range:
2 to 12
4 to 13
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Catalog No. Product Type Ligand Type pH Range
45-002-540 HiScreen Q Sepharose HP Column Quaternary Amine 2 to 12
45-002-542 HiScreen SP Sepharose HP Column Sulphopropyl 4 to 13
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Catalog No. 45-002-540 Supplier Cytiva Supplier No. 28950511
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Description

Designed for method optimization and parameter screening

  • Ready-to-use columns for convenient process development optimization and parameter screening
  • Highly cross-linked agarose matrix with an average bead size of 34μm
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 34μm
  • Max. flow velocity: <150cm/hr.
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm
  • Flow velocity: <150cm/hr.

HiScreen Q HP

  • Ionic capacity: 0.14 to 0.20mmol Cl-/mL
  • Ligand: quaternary amine
  • Ion exchanger type: strong anion exchanger
HiScreen SP HP
  • Ionic capacity: 0.15 to 0.20mmol Cl-/mL
  • Ligand: sulphopropyl
  • Ion exchanger type: strong cation exchanger

TRUSTED_SUSTAINABILITY

Specifications

Buffer 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS
Product Type HiScreen Q Sepharose HP Column
Bed Volume 4.7mL
Diameter (English) Inner 0.3 in.
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate <150 cm/hr.
Material Polypropylene
Ligand Type Quaternary Amine
Max. Pressure 3 bar
pH Range 2 to 12
Particle Size 45 to 165μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Ion Exchange Column
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
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