Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns

Designed for method optimization and parameter screening

Catalog Number	Mfr. No.	Product Type	Ligand Type	pH Range
45-002-540	Cytiva 28950511	HiScreen Q Sepharose HP column	Quaternary Amine	2 to 12
45-002-542	Cytiva 28950515	HiScreen SP Sepharose HP column	Sulphopropyl	4 to 13

Description

• Ready-to-use columns for convenient process development optimization and parameter screening
• Highly cross-linked agarose matrix with an average bead size of 34μm
• Bed height of 10cm designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

• Bed dimension: 7.7 × 100mm
• Matrix: 6% cross-linked agarose beads
• Average particle size: 34μm
• Max. flow velocity: <150cm/hr.
• Material (column hardware and column tube): polypropylene
• Column I.D.: 7.7mm
• Flow velocity: <150cm/hr.

HiScreen Q HP

• Ionic capacity: 0.14 to 0.20mmol Cl^-/mL
• Ligand: quaternary amine
• Ion exchanger type: strong anion exchanger

HiScreen SP HP

• Ionic capacity: 0.15 to 0.20mmol Cl^-/mL
• Ligand: sulphopropyl
• Ion exchanger type: strong cation exchanger

Specifications

• Bed Volume: 4.7mL
• Diameter (English) Inner: 0.3 in.
• Quantity: 1 x 4.7mL
• For Use With (Application): Optimization and parameter screening, ion exchange chromato graphy
• Height (Metric): 100mm
• Max. Pressure: 3 bar
• Storage Requirements: 4° to 30°C

• Buffer: 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS
• Diameter (Metric) Inner: 7.7mm
• Flow Rate: <150cm/hr.
• Height (English): 3.93 in.
• Material: Polypropylene
• Particle Size: 45 to 165μm
• Support Type: 6% Cross-linked Agarose