Cytiva HiScreen™ Sepharose™ Prepacked High Performance HIC Columns

Designed for method optimization and parameter screening

Catalog Number	Mfr. No.	Buffer	Product Type	Ligand Type	Support Type
45-002-545	Cytiva 28978242	1M NaOH, 1M Acetic Acid, 8M Urea, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 70% Ethanol, 3M Ammoniumsulfate, 1mM HCl, 30% Isopropanol, 2% SDS	HiScreen Butyl Sepharose HP column	Butyl	Cross-linked Agarose
45-002-543	Cytiva 28950516	1M NaOH, 1M Acetic Acid, 30% Aceto nitrile, 70% Ethanol, 30% Isopropanol, 6M Guanidine Hydrochloride, 8M Ure, 3M Ammoniumsulfate, Ionic Detergents, Non ionic Detergents, Polar Organic Solvents, 2% Sodium Dodectyl Sulfate	HiScreen Phenyl Sepharose HP column	Phenyl	6% Cross-linked Agarose

Description

• Ready-to-use columns for convenient process development optimization
• Standard aliphatic/aromatic Hydrophobic Interaction Chromatography media for capture and intermediate purification
• Bed height of 10cm designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

HiScreen Phenyl HP

• Degree of substitution: ∽25μmol phenyl/mL gel

HiScreen Butyl HP

• Degree of substitution: ∽50μmol phenyl/mL gel

Specifications

• Bed Volume: 4.7mL
• Diameter (Metric) Inner: 7.7mm
• Flow Rate: <150cm/hr.
• Height (English): 3.93 in.
• Material: Polypropylene
• Particle Size: 24 to 44μm
• Storage Requirements: 4° to 30°C

• Diameter (English) Inner: 0.3 in.
• Quantity: 1 x 4.7mL
• For Use With (Application): Convenient process development optimization parameter screening
• Height (Metric): 100mm
• Max. Pressure: 3 bar
• pH Range: 3 to 13