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Cytiva HisPrep Column

Optimize purification of histidine-tagged recombinant proteins.   Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.

Supplier:  Cytiva 28936551

Catalog No. 45-002-073

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  • Optimized for convenient scale-up purification of histidine-tagged proteins
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible leakage of Ni2+
  • Bed Volume 20ml
  • High binding capacity
  • Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium
  • Media:
  • BioProcess media
  • 90μm average particle size
  • Metrix 6% cross-linked agarose
  • pH stability working range 3-121
  • Metal ion capacity ∽15μmol Ni2+™/ml medium
  • Three years shelf life
  • Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3
  • Column:
  • Inside Diameter is 16mm
  • Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
  • Bed support Filter

  • Bed Dimensions 16 x 100mm
  • Flow rate <10ml/min1
  • Flow Velocity <300cm/h
  • Pressure Max. 1.5 bar



0.6 in.
4°C to 30°C
20 mL
<10 mL/min.
1.6 cm
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