Thermo Scientific™ SuperSignal™ West HisProbe Kit

Description

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Thermo Scientific™ HisProbe-HRP is an nickel (Ni²+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

• Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol

• Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies

• High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags

• Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Specifications

• Description: Pierce SuperSignal West HisProbe Kit
• Product Type: HisProbe Detection Kit
• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: HisProbe™, SuperSignal™
• Quantity: 685 mL
• Conjugate: HRP
• Substrate: Horseradish Peroxidase
• Reactivity: All species
• Sufficient For: 62 mini blots or 5000 sq cm membrane
• Content And Storage: Sufficient For: 62 mini blots or 5000 cm² membrane
  • HisProbe-HRP Conjugate, 2 mg
  • SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
  • SuperSignal West Pico Stable Peroxide Solution, 250 mL
  • Blocker BSA in TBS (10X), 125 mL
  • TBS Packs (each makes 500 mL), 10 packs
  • Surfact-Amps 20 Detergent Solution, 6 x 10 mL
  
  Store HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.