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Histone H4 (monomethyl R3) Rabbit, Clone: RM195, Abnova™

Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human Histone H4 (monomethyl R3).

Manufacturer:  Abnova Corporation MAB12784

Catalog No. 89-947-516

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq]



Histone H4 (monomethyl R3)
Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human Histone H4 (monomethyl R3).
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
100 ug
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
This antibody reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
ELISA, Western Blot
ELISA (0.2 ug/mL-1 ug/mL) Western Blot (0.5 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
A synthetic monomethyl peptide corresponding to residues surrounding R3 of Histone H4.
Protein A purification
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