Cytiva HisTrap™ FF Columns: 5 x 5mL

Catalog No.	Quantity
45-000-326	5 x 5 mL

Catalog No. 45-000-326 Supplier Cytiva Supplier No. 17525501

Description

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

• Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
• Column material: polypropylene
• Bed dimensions: 7 × 25mm
• Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
• Compatible with wide range of reducing agents, detergents, denaturants, and other additives
• Simple operation with syringe, perstaltic pump, or chromatographic system
• Columns may be connected in series to increase capacity

Medium

• Matrix: 6% cross-linked agarose
• Average particle size: 90μm
• pH stability working range: 3 to 12

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Specifications

• Bed Volume: 1 mL
• Diameter (English) Inner: 0.2 in.
• Diameter (Metric) Inner: 7 mm
• Description: HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
• Flow Rate: <4 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 6% Cross-linked Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Quantity: 5 x 5 mL
• Type: Pre-packed Column