Cytiva HisTrap™ FF Crude Prepacked Columns

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

• Prepacked with Ni Sepharose™ 6 Fast Flow media
• Binding capacity approximately 40mg/mL medium with negligible Ni²⁺ leakage
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
• 1 and 5mL columns

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 90μm
• pH stability working range: 3 to 12

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Bed Volume: 5 mL
• Diameter (English) Inner: 0.6 in.
• Diameter (Metric) Inner: 16 mm
• Description: HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
• Flow Rate: <20 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 6% Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Particle Size: 45 to 165 μm
• Quantity: 5 x 5 mL
• For Use With (Application): Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
• Type: Pre-packed Column