Cytiva HisTrap™ FF Crude Prepacked Columns

Catalog No.	Quantity	Flow Rate
45-000-335	5 x 5 mL	<20 mL/min.
45-000-000	60 mL	<4 mL/min.
45-000-507	1 mL	<4 mL/min.

Catalog No. 45-000-335

missing translation for 'mfr': Cytiva 17528601

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

• Prepacked with Ni Sepharose™ 6 Fast Flow media
• Binding capacity approximately 40mg/mL medium with negligible Ni²⁺ leakage
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
• 1 and 5mL columns

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 90μm
• pH stability working range: 3 to 12

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

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Specifications

• Bed Volume: 5 mL
• Diameter (English) Inner: 0.6 in.
• Diameter (Metric) Inner: 16 mm
• Description: HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
• Flow Rate: <20 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 6% Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Particle Size: 45 to 165 μm
• Quantity: 5 x 5 mL
• For Use With (Application): Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
• Type: Pre-packed Column