Cytiva HisTrap™ HP Prepacked Columns

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

• Prepacked with Ni Sepharose™ High Performance medium
• Negligible nickel ion leakage
• Pressure Max: 3 bar
• Column material: polypropylene
• 1 and 5mL columns
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Manual operation with syringe, pump or chromatography system

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 12

1mL columns

• Bed Dimensions: 7 x 25mm
• Flow velocity: 4mL/min.

5mL columns

• Bed Dimensions: 16 x 25mm
• Flow velocity: 5mL/min.

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Specifications

• Bed Volume: 1 mL
• Diameter (English) Inner: 0.2 in.
• Diameter (Metric) Inner: 7 mm
• Description: HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
• Flow Rate: 4 mL/min. (1 mL), 5 mL/min. (5 mL)
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Quantity: 5 x 1 mL
• Type: Pre-packed Column