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Cytiva HiTrap™ Capto™ IEX Selection Kit
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HiTrap Capto IEX Selection Kit
Description
- Consists of five HiTrap columns, prepacked with Capto ion exchange chromatography media with different characteristics
- For media screening; includes five 1mL HiTrap columns prepacked with Capto Q, Capto S, Capto DEAE, Capto MMC, Capto adhere, as well as connectors and instructions
- For optimization studies; buffer composition, pH, flow rate, sample loading and elution schemes can be optimized with small sample quantities prior to scale-up
- Capto Q is strong anion exchanger, Capto DEAE is weak anion exchanger, Capto S is strong cation exchanger
- Capto MMC and Capto adhere media have multimodal characteristics that can be used to solve specific purification problems
- Capto MMC is multimodal cation exchanger that is functional at high as well as at low ionic strength
- Capto adhere is multimodal strong anion exchanger mainly designed for post-protein A purification of monoclonal antibodies
- Prepacked columns and bulk media are available for larger scale preparative purifications
- Max. pressure (over packed bed during operation): 3 bar (42psi)
- Flow rate: <4mL/min.
- Complete packsize: 5 x 1mL
- Bed dimensions: 7 × 25mm
- Bed volume: 1mL
- pH Stability cleaning: 2 to 14
- Matrix: highly cross-linked agarose with dextran surface extender
- Average particle size: 90μm (d50V)
- pH Stability working range: 2 to 12
- Pressure/Flow specification: 0.3MPa at 700cm/hr., 1m diameter column, 20cm bed height
Column
- Column I.D.: 7mm
- Material (column hardware): polypropylene (PP)

Specifications
Specifications
Technique | Ion Exchange Chromatography |
Description | HiTrap Capto IEX Selection Kit |
Diameter (Metric) | 7 mm |
For Use With (Equipment) | Syringe, peristaltic pump and other chromatography systems such as AKTAdesign |
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