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  6. Cytiva HiTrap™ Chelating HP Columns

Cytiva HiTrap™ Chelating HP Columns

Catalog No. 45000061
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Quantity:
1 x 5mL
5 x 1mL
5 x 5mL

Description

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

TRUSTED_SUSTAINABILITY

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Bed Volume 5mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16mm
Quantity 1 x 5mL
Height (English) 0.98 in.
Height (Metric) 25mm
Flow Rate <20 mL/min.
Material Polypropylene
Ligand Type Iminodiacetic Acid
Max. Pressure 3 bar
pH Range 3 to 13
Particle Size 34μm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Affinity Column
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
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