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Cytiva HiTrap™ IEX HP Prepacked Columns

Catalog No. 45000514 Shop All Cytiva Products
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Volume (Metric):
1 mL
5 mL
Quantity:
5 Pk.
Each
Flow Rate:
<20 mL/min.
<4 mL/min.
Media Type:
Q Sepharose HP
SP Sepharose HP
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Catalog No. Volume (Metric) Quantity Flow Rate Media Type
45-000-514 1 mL Each <4 mL/min. SP Sepharose HP
45-000-191 1 mL 5 Pk. <4 mL/min. SP Sepharose HP
45-000-192 5 mL 5 Pk. <20 mL/min. SP Sepharose HP
45-000-193 1 mL 5 Pk. <4 mL/min. Q Sepharose HP
45-000-194 5 mL 5 Pk. <20 mL/min. Q Sepharose HP
45-000-515 1 mL Each <4 mL/min. Q Sepharose HP
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Catalog No. 45-000-514 Supplier Cytiva Supplier No. 29051324
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Description

Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.

 

  • Prepacked with Q or SP Sepharose High Performance
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series
  • Designed for use with syringe, peristaltic pump or chromatography system
  • Pressure Max: 3 bar
  • 1 and 5mL polypropylene columns
Media:
  • Particle size: 24 to 44μm
  • pH stability Working Range: 4 to 13

 

Q Sepharose (45-000-193, -194, 515)

  • Ligand: Quaternary amine
  • Ion Exchanger Type: Strong anion exchanger
  • Ionic Capacity: 0.14 to 0.20mmol Cl-/mL

SP Sepharose (45-000-191, -192, -514) 

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.15 to 0.20mmol H+/mL
1mL columns
  • Bed Dimensions: 7 x 25mm
  • Flow velocity: max 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: max 20mL/min.

 

TRUSTED_SUSTAINABILITY

Specifications

Description HiTrap SP HP, 1 x 1 mL
Diameter (English) Inner Column 0.2 in.
Flow Rate <4 mL/min.
For Use With (Application) HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification.
Media Type SP Sepharose HP
Content And Storage 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol
Diameter (Metric) Inner Column 7 mm
Volume (Metric) 1 mL
Quantity Each
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