Cytiva HiTrap™ MabSelect™ SuRe Prepacked Columns

Catalog No.	Bed Volume	Flow Rate
45-000-008	1 mL	<4 mL/min.
45-000-010	5 mL	<20 mL/min.
45-000-009	5 mL	<20 mL/min.
45-000-512	1 mL	<4 mL/min.

Catalog No. 45-000-008

Supplier: Cytiva 11003493

Description

Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs. MabSelect media are suited for the high flowrates and pressures required when scaling up.

• Oriented coupling of recombinant Protein A ligand to the agarose matrix via an engineered C-terminal cysteine increases IgG binding capacity over other protein A media
• High capacity, low ligand leakage, and high-flow base matrix allow purification of Mabs at process scale
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification
• Designed for use with a syringe, a peristaltic pump, or a chromatography system

MabSelect

• Allows at least five times higher flowrates than conventional cross-linked agarose of similar porosity
• Recombinant Protein A ligand promotes specificity of binding to F_c region of IgG; permits one-step purification

MabSelect Xtra™

• Same recombinant protein A ligand as MabSelect, but with a smaller particle size and greater porosity, for increased dynamic binding capacity
• Allows binding from samples with high expression levels of the monoclonal antibody
• Lowers overall production cost due to the possibility of processing concentrated feedstocks in fewer batches

MabSelect SuRe™ (Superior Resistance)

• Akali-tolerant protein A-derived ligand allows the use of up to 0.5M NaOH for cleaning-in-place (CIP)
• Permits significant cost savings and extension of cycles in regular production

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Specifications

• Bed Volume: 1 mL
• Binding Capacity: ≈35mg Human lgG/mL at 2.4 min. residence time
• Diameter (English) Inner: 0.27 in.
• Diameter (Metric) Inner: 0.7 cm
• Flow Rate: <4 mL/min.
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Quantity: 5 Pk.
• For Use With (Application): Chromatography System, Manual
• Type: Pre-packed Column
• Sample Volume: 1 mL