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Cytiva HiTrap™ NHS-Activated HP Columns

Catalog No. 45000137 Shop All Cytiva Products
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Quantity:
1 x 5mL
5 x 1mL
Flow Rate:
20 mL/min.
4 mL/min.
Bed Volume:
1mL
5mL
Diameter (Metric) Inner:
0.7cm
1.6cm
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Catalog No. Quantity Flow Rate Bed Volume Diameter (Metric) Inner
45-000-137 5 x 1mL 4 mL/min. 1mL 0.7cm
45-000-138 1 x 5mL 20 mL/min. 5mL 1.6cm
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Catalog No. 45-000-137 Supplier Cytiva Supplier No. 17071601
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Description

Includes

Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH

  • Designed for use with a syringe, peristaltic pump, or chromatography system
  • Degree of substitution: 10 μmol NHS/mL medium
  • Delivered in 100% isopropanol to protect the active groups
  • Stable in the pH range 3 to 12, depending upon ligand
  • Average particle size: 34μm

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TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 x 1mL
Flow Rate 4 mL/min.
Particle Size 34 μm
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Bed Volume 1mL
Product Type NHS-Activated HP Column
Diameter (Metric) Inner 0.7cm
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Height (English) 0.98 in.
Height (Metric) 2.5cm
Ligand Type 10μM NHS groups/mL medium
Material Polypropylene
Max. Pressure 42psi
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 8°C
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