Cytiva HiTrap™ Protein G HP Prepacked Columns

Catalog No.	Binding Capacity	Diameter (English) Inner Column	Flow Rate	Volume (Metric) Bed
45-000-053	25 mg Human IgG/Column	0.27 in.	<4 mL/min.	1 mL
45-000-055	125 mg Human IgG/Column	0.62 in.	>20 mL/min.	5 mL
45-000-054	25 mg Human IgG/Column	0.27 in.	>4 mL/min.	1 mL
45-000-056	125 mg Human IgG/Column	0.62 in.	>20 mL/min.	5 mL
45-000-504	25 mg Human IgG/Column	0.27 in.	<4 mL/min.	1 mL

Catalog No. 45-000-053 Supplier Cytiva Supplier No. 17040401

Description

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

• Prepacked with Protein G Sepharose High Performance
• Binding capacity: 25mg human IgG/column
• Maximum pressure: 3 bar
• Column material: polypropylene
• For use with syringe, peristaltic pump, or chromatography system
• 1 and 5mL columns

Media

• Ligand: Recombinant protein G lacking albumin-binding region
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 9

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

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Specifications

• Product Type: Pre-packed Columns
• Binding Capacity: 25 mg Human IgG/Column
• Diameter (Metric) Inner Column: 7 mm
• Diameter (English) Inner Column: 0.27 in.
• Flow Rate: <4 mL/min.
• Volume (Metric) Bed: 1 mL
• For Use With (Application): Designed for use with a syringe, peristaltic pump, or chromatography system
• Description: HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
• Storage Buffer: 20% Ethanol
• Content And Storage: 4°C to 30°C
• Quantity: 5 Pk.