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Cytiva™ HiTrap™ Protein Select™ Column

Catalog No. 09928219
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Affinity chromatography (tagged protein purification)
Column, Protein Select, Affinity, Affinity Chromatography, Chromatography
Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Includes

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Requires

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Affinity chromatography resin for purifying any recombinant protein that does not have an affinity binding partner.

Cytiva™ Protein Select™ resin is an affinity chromatography resin for purifying any recombinant protein that does not have an affinity binding partner.

  • Simple affinity purification protocol for recombinant proteins.
  • One-step purification and tag cleavage without the need for proteases or other elution reagents.
  • Delivers a highly pure, native protein with no remaining tag amino acids after tag cleavage.
  • Can be processed under mild conditions,which means no exposure to conditions that cause protein aggregation or loss of biological activity.
  • Flexible,enabling the use in multiple expression systems.

About

  • Affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag.
  • Bed dimensions: 0.7 x 2.5 cm
  • This technology simplifies tagged protein purification when used in research and standardizes purification during process development of any protein that does not have an affinity binding partner.

A Simple Purification Protocol

  • Purifying a protein tagged with Cytiva™ Protein Select™ tag is simple.
  • One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal.
  • The pure protein obtained is in its native state with no traces of the tag amino acids.
  • The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.

Formats for Research Through to Commerican Manufacturing

  • Cytiva™ Protein Select™ resin is available in 25 mL, 100 mL, 500 mL, 1 L, and 5 L packs of bulk resins and in HiTrap™ 1 mL and 5 mL columns, which are well suited for research, process development and scaling up to commercial scales.
  • Packing methods for Tricorn™, HiScale™, or AxiChrom™ chromatography columns are available.

Order Info

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Certifications

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Compliance

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Warranty and Services

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

Column, Protein Select, Affinity, Affinity Chromatography, Chromatography

TRUSTED_SUSTAINABILITY

Specifications

Type Column
Quantity 1 Column
Flow Rate 4 mL/min. Max
Volume (Metric) 1 mL
Diameter (Metric) 7 mm
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