Novus Biologicals™ HIV-1 gp120 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Immunodeficiency Virus type 1 (HIV-1) gp120.

Specifications

• Assay Range: 46.88 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Immunodeficiency Virus type 1 (HIV-1) gp120.
• Target Species: Virus
• For Use With (Application): Sandwich ELISA
• Gene Alias: Envelope surface glycoprotein gp120, gp120, gp120 glycoprotein, Human immunodeficiency virus 1, SU, Surface protein
• Gene ID (Entrez): 155971
• Gene Symbol: env
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human HIV-1 gp120 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of rabbit anti-Human HIV-1 gp120 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 165 ng of recombinant Human HIV-1 gp120.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Virology Bacteria and Parasites
• Storage Requirements: Storage is content dependent.
• Target: HIV-1 gp120
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.