Invitrogen™ HIV1 Tat Monoclonal Antibody (7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA171509

Catalog No. MA171509

Description

This antibody reacts with HIV-1 Tat BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1 subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). It has been mapped to amino acids 6-12 (PRLEPWK - B subtype a.a. consensus). MA1-71509 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat. This clone may also be referred to as 7 (02-002).

HIV-1 Tat (Trans-Activator of Transcription) consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.

Specifications

• Antigen: HIV1 Tat
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, ELISA, Western Blot
• Clone: 7
• Conjugate: Unconjugated
• Gene Alias: HIV-1 Tat
• Immunogen: Recombinant Tat protein of HIV-1 BH10 purified from E. coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid