Invitrogen™ HIV1 Tat Monoclonal Antibody (7)

Catalog No.	Quantity
MA171509	100 μg

Catalog No. MA171509 Supplier Invitrogen™ Supplier No. MA171509

Description

Mouse Monoclonal Antibody

This antibody reacts with HIV-1 Tat BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1 subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). It has been mapped to amino acids 6-12 (PRLEPWK - B subtype a.a. consensus). MA1-71509 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat. This clone may also be referred to as 7 (02-002).

HIV-1 Tat ('Trans-Activator of Transcription') consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.

Specifications

• Antigen: HIV1 Tat
• Applications: ChIP Assay, ELISA, Western Blot
• Classification: Monoclonal
• Clone: 7
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Alias: HIV-1 Tat
• Host Species: Mouse
• Immunogen: Recombinant Tat protein of HIV-1 BH10 purified from E. coli.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Virus
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1