HLA-A25/HLA-AW32 Mouse anti-Human, Clone: SPM417, Abnova™

Mouse monoclonal antibody raised against native human HLA-A25/HLA-Aw32.

Manufacturer:  Abnova Corporation MAB13071

Catalog No. 89-934-258

Description

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq]

Specifications

• Antigen: HLA-A25/HLA-AW32
• Clone: SPM417
• Description: Mouse monoclonal antibody raised against native human HLA-A25/HLA-Aw32.
• Formulation: In 10mM PBS (0.05% BSA, 0.05% sodium azide).
• Gene Alias: HLAA
• Host Species: Mouse
• Isotype: IgG2a, kappa
• Quantity: 100 ug
• Storage Requirements: Store at 4°C.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: This monoclonal antibody reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This antibody is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
• Conjugate: Unconjugated
• Dilution: Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
• Format: Liquid
• Gene Symbols: HLA-A
• Immunogen: Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, DR2, DRw10.
• Purification Method: Protein A/G purification
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3105/3106/3107
• Target Species: Human